Early diagnosis of hepatitis A virus-micro-blotting immuno-binding assay

Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2

Hepatitis C Virus (HCV) encodes two envelope glycoproteins, E1 and E2. Our previous work selected a specific aptamer ZE2, which could bind to E2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting HCV. In this study, the binding sites between E2 and aptamer ZE2 were further explored. E2 was truncated to 15...

Quantitative Bacterial Micro-Assay for Rapid Diagnosis of Galactosemia: Application in Galactosemia Neonatal Screening

In the present study a new economic and rapid bacterial micro-assay for simultaneous detection and quantitative measurement of serum galactose was developed. Analysis of the standard curve showed a linearity range for galactose from 2 mg/dL to 180 mg/dL with a regression equation of Y = 0.013X ? 0.083; R² = 0.962. The advantage of the method is its ability to measure serum galactose quantitativ...

The Use of indirect Immunoperoxidase Assay in Diagnosis of Type A (H9N2) Avian Influenza Virus Antigen on Frozen Tissue Sections

Diagnosis of Acute Hepatitis C Virus

Recent findings A rising incidence of acute hepatitis C virus (HCV) in HIV-infected MSM has been observed since 2000 in Europe, Australia, USA and Asia. Transmission appears to occur through the permucosal rather than the more usual parenteral route. Although often multifactorial, permucosal risk factors can be classified as behavioural (sexual practices and mucosally administered drugs) and bi...

A case of dermatitis artefacta: Clues which help early diagnosis

A 26-year-old married female presented with a complaint of recurrent multiple ulcerative lesions on her body. After complete history and physical examination, a diagnosis of dermatitis artefacta was made. Dermatitis artefacta remains undiagnosed for quite some time because of its atypical skin changes, diverse methods used for producing skin lesions, lack of awareness about the disorder on the ...